Twist Bioscience Corporation announced the expansion of its high-throughput antibody portfolio with the launch of a CHO cell line for Twist Express Antibodies. Twist also offers HEK293 expression Twist Express Antibodies. Using its high-throughput antibody production workflow, Twist can generate large quantities of small-scale distinct antibodies for candidate and drug discovery screening. Antibodies are created from user-defined sequences using the customer?s expression system of choice, either CHO or HEK293. Twist Express Antibodies are sequence-perfect and high quality, delivered in as few as 10 business days. Twist Express Antibodies, CHO, are developed using a high-throughput antibody production workflow that yields IgG antibodies in quantities ranging from 15 µg up to 3,000 µg. The antibodies are made using sequence-verified perfect clonal genes for high quality. Twist also provides yield data on each antibody sequence to be used as a screening tool for candidate selection as well as control yield data to provide peace of mind and confidence of successful transfection and protein production. Turnaround time for Twist Express Antibodies, CHO, starts at 13 business days.
Twist Express Antibodies provide a comprehensive and efficient solution to antibody production and purification. The process begins with a customer entering antibody sequences into Twist?s eCommerce ordering platform, where bioinformatic screening and gene scoring is used to filter out potentially non-viable sequences. From there, synthetic DNA is generated, which is either cloned into one of Twist?s IgG scaffold vectors, or a vector of the customer?s choice. Twist?s silicon-based DNA synthesis platform enables the simultaneous production of up to thousands of antibody genes, which enables flexible production of tens to thousands of distinct antibodies. Twist sequences the antibody genes to ensure that the precise order has been cloned correctly, every time. These antibodies are then produced in CHO or HEK293 cells, resin purified, and have a turnaround time starting at 10 business days for HEK293 and 13 business days for CHO.